Biomarkers play a crucial role in disease diagnosis, prognosis, and treatment monitoring. This study focuses on developing non-enzymatic electrochemical sensors for the detection of glucose. It explores nickel-based electrodes, comparing Nickel Flag Electrode (NFE), Nickel Wire Electrode (NWE), and Nickel-Graphene-Carbon (NiGC/GCE) composite electrodes. The NFE sensor exhibited high sensitivity (9650 &muAmM⁻¹cm⁻²) with a 100 &muM detection limit, while the NWE sensor showed lower sensitivity (1460 &muAmM⁻¹cm⁻²) with detection limit (0.5 &muM). The NiGC/GCE sensor, leveraging graphene/carbon synergy, achieved exceptional sensitivity (8890 &muAmM⁻¹cm⁻²) and a 0.17 &muM detection limit. Electrochemical analyses confirmed strong stability and selectivity. The NiGC/GCE sensors superior performance highlights its potential for miniaturized, cost-effective glucose monitoring. These findings promote Ni-based sensors as viable alternatives to noble metals, advancing affordable and scalable diabetes management solutions.