Culture-dependent and Culture-independent analysis identified a higher abundance of phylum Ascomycota, explicitly belonging to genera Fusarium, Aspergillus, and Zopfiella in the marine regions of east coast. Marine fungi play a pivotal role in bioremediation of organic and inorganic pollutants. In addition, biofilms formed by filamentous fungi show great potential in xenobiotic degradation. The structural and functional properties of fungal biofilm and associated extracellular matrix (ECM) facilitate the breakdown and assimilation of organic matter, while strong adherence to the ECM functional groups leads to effective sequestration of heavy metals. Furthermore, extracellular lignocellulolytic enzymes released by the manglicolous fungus Trichoderma sp. catalyzed the degradation process of complex organic compounds. Similarly, Aspergillus niger showcases immense metabolic potentials, such as high cell wall binding, biomass production, and synthesis of novel enzymes and bioactive compounds, fostering their diverse biotechnological applications. The synthesis of myconanoparticles represents a sustainable approach to harness the unique properties of these fungi for environmental remediation.