Course Details
Subject {L-T-P / C} : BM4104 : Structural Biology { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Subhankar Paul
Syllabus
Introduction, Interaction in biology systems, Structure of Biomolecules: protein and nucleic
acids, Secondary, tertiary and quaternary structure of protein, Primary and secondary structure
of RNA and DNA, Method of conformational analysis and prediction of conformation,
Thermodynamics and kinetics of conformational transition of proteins, Protein folding,
techniques for studying Macromolecular structure, Ultra centrifugation Sedimentation velocity
and equilibrium, determination of molecular weights, Electron microscopy, UV Visible
Spectroscopy, Fluorescence Spectroscopy, Circular Dichroism Spectroscopy, Symmetry,
space group crystal lattices, brag’s law in real & reciprocal space, Nuclear Magnetic
Resonance.
Course Objectives
- To understand protein structure and function
- To learn protein folding process in vitro and in vivo
- To learn the analysis of protein conformational change in vitro
- To learn about various standard techniques to understand protein there dimensional structure.
Course Outcomes
On completion of this course, the student will be able to: <br />1. Understand the evolution of protein structural motifs and domains and associate this with function <br />2. Use on-line structural databases and tools to predict the properties, structure and function of proteins. <br />3.Understand and explain enzyme mechanisms in a structural context. <br />4. Describe mechanisms of protein folding and the roles of natively unstructured proteins in biology. <br />5. Understand how cross-talk between proteins and post-translational protein modifications (e.g. phosphorylation, ubiquitination) facilitate information processing in cells.
Essential Reading
- . D. L. Nelson and M. M. Cox, Lehninger, Principles of Biochemistry, W. H. Freeman,, Prentice Hall , Fourth Edition, 2004.
- K. Evan Holde, C. Johnson and P. S. Ho,, Principles of Physical Biochemistry, Prentice Hall, Second Edition, 2005, Prentice Hall , Second Edition, 2005.
Supplementary Reading
- P R. Bergethon, The Physical Basis of Biochemistry, The Foundations of Molecular Biophysics, Springer , Corrected Edition, 2000
- K. Evan Holde, C. Johnson and P. S. Ho,, Principles of Physical Biochemistry, Prentice Hall, Second Edition, 2005, Prentice Hall , Second Edition, 2005.