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TIIR Building, NIT Campus, Rourkela, Odisha, 769008

Inverted Microscope

Instrument Images
LS0307

INSTRUMENT INCHARGE

Prof. Bijesh Kumar Biswal

Associate Professor

Cancer and Stem Cell Biology

BASIC INFORMATION

  • Make & Model : Olympus CX23
  • Department : Life Science
  • Location : MN241B
  • Category : Category - III
  • Status : Functional

PRINCIPLE:

In an inverted microscope, the source for transmitted light and the condenser are placed on the top of the stage, pointing down toward the stage. The objectives are located below the stage pointing up. The cells are observed through the bottom of the cell culture vessel.

APPLICATION:

Inverted microscopy is a very popular technique for live cell imaging. Here, living cells are observed through the bottom of a cell culture vessel. This technique has several advantages over upright microscopy. Most cells naturally sink to the bottom of the vessel and—if no suspension cells are used—adhere to the surface, meaning that they are spread across one focal plane. When growing in vessels that are suitable for inverted microscopy, cells have access to larger amounts of medium than if they were squeezed between a coverslip and a slide. In addition, sample access from the top is possible, for medium exchange or micropipettes, for example. Another very important advantage is sterility: as there is no contact between the objective and the sample, sterile working conditions are guaranteed.

EXPERIMENTS & CHARGES:

EXPERIMENT - 1

  • Name of Experiment : Inverted microscopy
  • Condition of Analysis : As per MSDS & Cover Letter
  • Service Units : 1 Hour
  • Academic & Educational Institution Charges : ₹ 236.00
  • R & D National Laboratory Charges : ₹ 590.00
  • Industry Charges : ₹ 590.00
  • International Charges : ₹ 944.00
  • Available for Sample Booking : Yes
  • Remarks :