Monday - Friday 09:00 hr to 18:00 hr
TIIR Building, NIT Campus, Rourkela, Odisha, 769008

Programmable Controlled Rate Freezer

Instrument Images
BM0010

INSTRUMENT INCHARGE

Prof.(Ms.) Krishna Pramanik

Professor

Nanobiotechnology / Nanomedicine, Biomaterial & Tissue engineering, Stem cell Biology

BASIC INFORMATION

  • Make & Model : Planar Kryo-560-16 & 360-1.7 programmable control rate freezer cooling down to -180oC.
  • Department : Biotechnology and Medical Engineering
  • Location : Tissue Engineering Lab-II (stem cell culture)
  • Category : Category - III
  • Status : Functional

PRINCIPLE:

The Planar Kryo-560-16 and Planar Kryo-360-1.7 programmable control rate freezers function based on precise refrigeration technology designed to control temperature changes at very low rates. The main principles of operation include: Precise Temperature Control:These freezers use liquid nitrogen (LN2) or mechanical refrigeration systems to maintain ultra-low temperatures. The temperature inside the freezer can be precisely controlled, allowing the freezer to reach and maintain temperatures as low as -180°C. Programmable controls allow for the user to set specific cooling rates, cooling profiles, and target temperatures to suit different cryopreservation and cooling applications. Programmable Cooling and Heating:Users can input specific cooling profiles (e.g., cooling rates, temperature set points, and duration) into the freezer’s system. These profiles enable gradual and controlled freezing, which is essential for preventing cell damage during cryopreservation. The cooling rate is programmable and adjustable, which allows the freezer to cool samples at a rate that ensures maximum cell viability. Cooling can start at a high rate, but as the sample temperature approaches the target temperature, the rate can slow down to prevent thermal shock to the samples. Cryopreservation and Ice Nucleation: Controlled cooling minimizes the risk of ice crystal formation inside cells and tissues, which could damage them. Cryopreservation requires that cells be cooled at a specific rate to avoid damaging their structure, which is facilitated by the programmable nature of the freezer. The slow freezing process allows intracellular water to slowly move out of cells, avoiding the formation of ice crystals, which could rupture cell membranes. Automatic Data Logging and Monitoring: These freezers are equipped with data logging systems that record temperature changes throughout the freezing cycle. This ensures that the freezing process is monitored and controlled, offering full traceability and reproducibility. They often feature alarms and visual indicators for any deviations from set parameters, including temperature excursions or equipment failure, ensuring sample safety. Energy Efficiency:These units are designed to be energy-efficient while providing consistent cooling, minimizing the energy consumption during the freezing process while maximizing performance and sample protection. Insulation: Both models are equipped with high-quality insulation to ensure minimal heat exchange with the external environment, keeping the ultra-low temperature stable.

APPLICATION:

Cryopreservation: Used extensively for the storage of biological samples, such as stem cells, sperm, eggs, embryos, and tissues. These sensitive biological materials require slow, controlled freezing to avoid the formation of ice crystals that can damage the cells. Used in fertility clinics, biobanks, research institutions, and pharmaceutical companies. Controlled Freezing for Biological Research:The freezing and thawing process is critical in biological research where cells or biological agents need to be preserved for long periods before future use. In cryobiology and gene therapy, maintaining cell viability after freezing is crucial for various studies and experiments. Biotechnology and Pharmaceutical Applications: Used in the preservation of enzymes, vaccines, and antibodies for pharmaceutical and biotech industries. Essential for maintaining the integrity and activity of proteins or compounds that are temperature-sensitive. Storage of Blood Products: Critical for the long-term storage of blood plasma, red blood cells, and other blood components. Often employed in blood banks and hospitals to ensure the viability of blood products until they are needed. Microbial and Cellular Preservation: Used for the preservation of microbial cultures, bacterial strains, and other biological entities at ultra-low temperatures. Regenerative Medicine: Employed in preserving human cells for regenerative therapies or treatments that rely on cryopreservation.

EXPERIMENTS & CHARGES:

EXPERIMENT - 1

  • Name of Experiment : Biological Sample Preservation
  • Condition of Analysis : As per MSDS and Cover letter
  • Service Units : 1 Sample
  • Academic & Educational Institution Charges : ₹ 1,180.00
  • R & D National Laboratory Charges : ₹ 2,360.00
  • Industry Charges : ₹ 3,540.00
  • International Charges : ₹ 5,900.00
  • Available for Sample Booking : Yes
  • Remarks :