UV-Visible Absorption: In this mode, the instrument measures the absorption of ultraviolet (UV) and visible light by a sample. The sample absorbs light at specific wavelengths, and the amount of light absorbed provides information about the chemical composition and concentration of the sample.
Fluorescence: In fluorescence mode, the instrument excites a sample with light at a specific wavelength, and then detects the emitted light at a longer wavelength. This allows for the study of fluorescence characteristics of the sample, which can be used to study molecular interactions, concentrations, and properties of biomolecules.