R & D Resourses

High Performance Liquid Chromatography

BM0225

INSTRUMENT INCHARGE

Amit Biswas

Associate Professor

Biomaterials, Surface Engineering, Corrosion and oxidation of materials

BASIC INFORMATION

Make & Model : Agilent 1200 Infinity Series Agilent Technologies Mfg GmbH&Co.KG Reverse Phase Liquid Chromatography
Department : Biotechnology and Medical Engineering
Location : Bimaterial Lab Room No-117
Category : Category - II
Status : Functional

BOOKING CLOSED

PRINCIPLE:

The Agilent 1200 Infinity Series HPLC (High-Performance Liquid Chromatography) works on the principle of separation of mixtures based on their interaction with a stationary phase (column material) and a mobile phase (solvent). In reverse phase chromatography, the stationary phase is typically non-polar (e.g., C18), while the mobile phase is polar (e.g., water and organic solvents). Compounds in the sample are separated based on their hydrophobicity; more hydrophobic substances interact strongly with the stationary phase and move more slowly through the column.

APPLICATION:

Pharmaceutical Industry: For analyzing drug formulations, testing purity, and quantifying active pharmaceutical ingredients (APIs). Environmental Testing: Detecting pollutants, pesticides, and heavy metals in water, soil, or air samples. Food and Beverage: Analyzing flavors, preservatives, contaminants, and nutritional content. Biotechnology: Used in protein purification, biomolecule analysis, and peptide mapping. Clinical Diagnostics: For metabolic testing, blood and urine analysis, and diagnostic assays

EXPERIMENTS & CHARGES:

EXPERIMENT - 1

Name of Experiment : Liquid chromatography of reverse phase sample
Condition of Analysis : As per MSDS document and covering letter
Service Units : 1 Hour
Academic & Educational Institution Charges : ₹ 1,180.00
R & D National Laboratory Charges : ₹ 1,770.00
Industry Charges : ₹ 2,360.00
International Charges : ₹ 5,900.00
Available for Sample Booking : No
Remarks :