Environmental Scanning Electron Microscope

Instrument Images
BM0261

INSTRUMENT INCHARGE

Prof.(Ms.) Krishna Pramanik

Professor

Nanobiotechnology / Nanomedicine, Biomaterial & Tissue engineering, Stem cell Biology

BASIC INFORMATION

  • Make & Model : FEI Quanta FEG 250.
  • Department : Biotechnology and Medical Engineering
  • Location : ESEM Lab,Tissue Engineering ( Room No. 109)
  • Category : Category - I
  • Status : Non-Functional

PRINCIPLE:

An ESEM employs a scanned electron beam and electromagnetic lenses to focus and direct the beam on the specimen surface in an identical way as a conventional SEM. A very small focused electron spot (probe) is scanned in a raster form over a small specimen area. The beam electrons interact with the specimen surface layer and produce various signals (information) that are collected with appropriate detectors. The output of these detectors modulates, via appropriate electronics, the screen of a monitor to form an image that corresponds to the small raster and information, pixel by pixel, emanating from the specimen surface.

APPLICATION:

ESEM offers an efficient imaging method for histology and pathology. In ESEM, samples are not dehydrated or embedded and can be placed in the microscope after a very few and simple preparation steps. Backscattered electron contrast, used for imaging wet samples under water vapour conditions, reveals high contrast sample details. The resulting images help to understand 3D architecture of different types of tissue without any serial sectioning and reconstruction. In Tissue Engineering And pathology, ESEM imaging can be used to provide direct evidence of pathological tissue conditions

EXPERIMENTS & CHARGES:

EXPERIMENT - 1

  • Name of Experiment : Material sample Imaging (Nonbiological Sample)
  • Condition of Analysis : As per MSDS and Cover letter
  • Service Units : 1 Sample
  • Academic & Educational Institution Charges : ₹ 944.00
  • R & D National Laboratory Charges : ₹ 1,180.00
  • Industry Charges : ₹ 1,416.00
  • International Charges : ₹ 1,770.00
  • Available for Sample Booking : No
  • Remarks :

EXPERIMENT - 2

  • Name of Experiment : Material sample Imaging (Biological Sample)
  • Condition of Analysis : As per MSDS and Cover letter
  • Service Units : 1 Sample
  • Academic & Educational Institution Charges : ₹ 1,180.00
  • R & D National Laboratory Charges : ₹ 1,416.00
  • Industry Charges : ₹ 1,770.00
  • International Charges : ₹ 2,124.00
  • Available for Sample Booking : No
  • Remarks :