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To observe the sample through a fluorescence microscope, it should first be labeled with fluorescent dyes/substances known as fluorophores. Higher energy shorter wavelength lights (UV rays or blue light) generated from mercury vapor arc lamp pass through the excitation filter. The excitation filter allows only the short wavelength of light to pass through and removes all other non-specific wavelengths of light. The filtered light is reflected by the dichroic filter and falls on the fluorophore-labeled sample. The fluorochrome absorbs shorter wavelength rays and emits rays of longer wavelength (lower energy) that pass through the emission filter. The emission filter blocks (suppresses) any residual excitation light and passes the desired longer emission wavelengths to the detector. Thus, the microscope forms The Axiovert Fluorescence Microscope from Carl Zeiss operates based on the principle of fluorescence microscopy, which allows for the observation of samples that fluoresce under ultraviolet (UV) light or specific wavelengths. The microscope illuminates the sample with light at a specific wavelength, exciting the fluorophores (molecules that emit light upon being excited). These fluorophores emit light at a longer wavelength, which is captured by the microscope’s detection system (often equipped with a camera or photodetector). This provides high contrast and resolution for the visualization of specific components within the sample.