Course Details
Subject {L-T-P / C} : BM3324 : Enzymology and Protein Engineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Nandini Sarkar
Syllabus
Enzyme classification and co-factors, Kinetics of enzymatic reactions, Types of enzyme inhibitors and their mechanism of action, Enzyme immobilization and its application, Introduction and scope of Protein Engineering, Different approaches of protein engineering, Random mutagenesis, Mutagenesis by rational design, Protein sequencing, Effect of mutation on protein structure, stability and folding, Applications of protein engineering, Examples of commercially used engineered proteins.
Course Objectives
- Gaining in-depth knowledge on enzyme activity as well as inhibition mechanism.
- Learning different methods of enzyme immobilization and pros and cons of each method.
- Learning different approaches of incorporating mutations in proteins and studying effects on the mutations on protein folding and stability.
- Learning different methods of biophysical characterization of engineered proteins, Examples of commercially used engineered proteins.
Course Outcomes
1. Understanding mechanism of enzyme action and inhibition and studying effects of various external factors on enzyme activity. <br />2. Understanding various enzyme immobilization methods and their applications. <br />3. Review of factors significant for protein folding processes and stability. <br />4. Understanding of advanced biophysical techniques for protein analysis, including the capacity to discuss their relative merits and interpret data from those techniques. <br />5. Understanding the basics of protein engineering and its applications.
Essential Reading
- G. Walsh, Proteins: Biochemistry and Biotechnology, Wiley Blackwell
- Richard M. Twyman, Principles of Proteomics, Taylor & Francis
Supplementary Reading
- S. Park and J. Cochran, Protein Engineering and Design, CRC Press
- Nelson D. and Cox. M., Lehninger: Principles of Biochemistry, W.H. Freeman & Co.