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Characterization and cadmium-resistant gene expression of biofilm forming marine bacterium Pseudomonas aeruginosa JP-11. Prof. Surajit Das and Jaya Chakraborty, Department of Life Science.

B
iofilm forming marine bacterium Pseudomonas aeruginosa JP-11 resisted up to 1000 ppm of cadmium (Cd) as cadmium chloride. Biomass and extracellular polymeric substances (EPS) secreted by the cells effectively removed 58.760±10.62% and 29.544±8.02% of Cd, respectively. The integrated density of the
biofilm-EPS observed under fluorescence microscope changed significantly (P≤0.05) in presence of 50, 250, 450, 650 and 850 ppm Cd. ATR-FTIR spectroscopy depicts the presence of sulphydryl group (-SH) within the EPS, responsible for binding with cadmium. On exposure to Cd at 100, 500 and 1000 ppm, the expression profiles of cadmium resistance gene (czcABC) in the isolate showed an up-regulation of 3.52, 17 and 24 fold, respectively. Down-regulation was observed with variation in the optimum pH (6) and salinity (20 g/l) level. More in Environ Sci Pollut Res, 21, 14188–14201 (2014). DOI 10.1007/s11356-014-3308-7 (List of Research)

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