Course Details
Subject {L-T-P / C} : BM4006 : Metabolic Engineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Kasturi Dutta
Syllabus
Basic concepts of Metabolic Engineering, Overview of cellular metabolism, Different models for cellular reactions,Methods for metabolic characterization: genome, transcriptome, proteome, metabolome, fluxome Comprehensive models for cellular reactionsCoordination of metabolic reactions: Feedback inhibition, Energy charge, Multigene networks, Metabolic regulation network at enzyme level and whole cell level, Examples of metabolic pathway manipulations, Metabolic pathway synthesis algorithms, Metabolic flux analysis and its applications, Methods for experimental determination of metabolic fluxes,Metabolite Balancing, Tracer Experiments, MS and NMR in labelling measurement., Analysis of Metabolic control analysis (MCA), Determination of Flux control coefficients, MCA of Linear and Branched pathways, Thermodynamics of cellular processes, Metabolic design: Gene amplification, Gene-disruption, Randomized and targeted strain development, New concepts for quantitative bioprocess research and development.
Course Objectives
- Knowledge of stoichiometry and energetics of metabolism.
Course Outcomes
1. Knowledge of stoichiometry and energetics of metabolism. <br />2. To apply practical applications of metabolic engineering in chemical, energy, medical and environmental fields.
Essential Reading
- Stephanopoulas, G, et al.,, Introduction to Metabolic engineering – Principles and Methodologies, Elsevier Scienc
- S. Y. Lee, E.T. Papoutsakis, Metabolic Engineering, Marcel Dekker
Supplementary Reading
- F.David, Understanding the Control of Metabolism,, Portland Press
- R. Heinrich and S., Schuster, The regulation of cellular systems, Springer Science & Business Media