Course Details
Subject {L-T-P / C} : BM4707 : Downstream Processing and Bioseparation Laboratory { 0-0-3 / 2}
Subject Nature : Practical
Coordinator : Prof. Kasturi Dutta
Syllabus
Separation of proteins from mixture using ammonium sulphate precipitation method, separation of biomolecules using dialysis, determination of filtration efficiency of a filter press, separation of carbohydrates from non-fat powdered milk, separation of phenolic bioactive compounds using silica-gel column chromatography, separation of amino acids using thin-layer chromatography, isolation of ethanol from fermentation broth using distillation, separation of
proteins using SDS-PAGE gel electrophoresis, determination of void volumes of gel filtration column, fractionation of mixture of carbohydrates using gel filtration chromatography, extraction of glycoproteins from bacterial cell lysate by affinity chromatography
Course Objectives
- Hands on experience of basic downstream processes
Course Outcomes
working experience of basic downstream processes
Essential Reading
- P.A. Belter, E.L. Cussler and Wei-Houhu, Bioseparations – Downstream Processing For Biotechnology,, Wiley Interscience Pub
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Supplementary Reading
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