Course Details
Subject {L-T-P / C} : BM4105 : Biomolecular Engineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Bibhukalyan Prasad Nayak
Syllabus
Biomolecules definition, their function in cells - Proteins,Nucleic acid, starch and their basic
structure. Various forces that determine biomolecule structure. Mechanisms of protein folding,
characterization of folding pathways. Melting points and importance, biomolecules stability.
Denaturants, determination of biomolecule structure by various spectroscopic techniques.
Background and basic principles, Absorption and Fluorescence, Circular Dichroism, FTRaman, FT-IR, NMR, X-ray crystallography, DSC. Protein denaturation, aggregation and
gelation. Gene cloning: Restriction enzymes in gene cloning, PCR technology for gene/DNA
detection, cDNA, usages of plasm id and phagesas vectors Model vectors for eukaryotes Viruses, Gene libraries Use of marker genes Medicine related applications i.e. commercial
synthesis of hormones and vaccines. Microbial applications i.e.large scale preparation of
organic chemicals, large scale biomolecules production and their other industrial applications.
Course Objectives
- To enable students learn analytical technologies and the principles involved.
Course Outcomes
The course will prepare the students ready to work in biomolecule synthethis labs/industries.
Essential Reading
- J.L. Clel, Protein Engineering: Principles and Practice, Wiley
- S.T. Desmond, An Introduction to Genetic Engineering, Cambridge University Press
Supplementary Reading
- A. Divan, Tools and Techniques in Biomolecular Science, Oxford
- No thanks, No thanks, No thanks