Course Details
Subject {L-T-P / C} : BM4711 : Plant Biotechnology Laboratory { 0-0-2 / 1}
Subject Nature : Practical
Coordinator : Prof. Nivedita Patra
Syllabus
Tissue culture media preparation Callus induction Hairy root induction Plant genomic DNA isolation RNA isolation Reverse transcriptase PCR Genetic transformation of plants effect of cultivation conditions, Elicitation of secondary metabolites Permeabilization In-situ product extraction Immobilization of plant cells Scale-up of plant cell cultivation in a bioreactor.
Course Objectives
- To train the students in plant biotechnology techniques
Course Outcomes
1. Extensive knowledge of plant tissue culture techniques. <br />2. In-depth understanding of new methods for plant micropropagation. <br />3. Understanding the need for innovation for large scale plant secondary metabolite production.
Essential Reading
- M.J. Chrispeels and D.E. Sadava, Plant, Genes and Crop Biotechnology, American Society of Plant Biologists , 2000
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Supplementary Reading
- P.K. Chawla, Textbook of Plant Biotechnology, Oxford and IBH, New Delhi , 2000
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