Course Details
Subject {L-T-P / C} : BM0008 : Biotechnology Laboratory { 0-0-2 / 1}
Subject Nature : Practical
Coordinator : Prof. Balasubramanian P
Syllabus
Isolation of microflora (bacteria, fungi, mold and pollen) from various environmental medium.
Study of growth cycle of bacteria in a batch cultivation system. Determination of specific growth rate of bacteria in batch reactor and CSTR. Demonstration of design expert and xlstat softwares. Demonstration of RSM in matlab. Electrophoresis of Protein, PAGE, SDS-PAGE, Agarose electrophoresis of nucleic acid, Development of enzyme assays and quantification of enzyme activity and specific activity. Experiments on Biodegradation, Biosorption and Bioleaching. Physicochemical and biological analysis of air, water and soil.
Course Objectives
- To get familiarize with the laboratory protocols on various aspects of biotechnology
Course Outcomes
On successful completion of this course on Biotechnology Laboratory, students could have an understanding on the analytical concepts on various aspects of biotechnology. Specifically, the students could able to isolate, cultivate and quantify the growth of a microbe in a reactor and its real-time applications.
Essential Reading
- Verma Ashish S, Laboratory Manual for Biotechnology Students, S Chand Company , 2014
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Supplementary Reading
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