Course Details

Subject {L-T-P / C} : BM2705 : Cell and Molecular Biology Laboratory { 0-0-3 / 2}

Subject Nature : Practical

Coordinator : Prof. Angana Sarkar

Syllabus

Welcome to laboratory, introduction to laboratory equipment and learning safety and aseptic measures while working on wet bench/clean room, Basic principle of microscopy: hands on experience with light and inverted fluorescence microscope, working with magnification, resolution and visibility, aberrations, control of illumination in the microscope, Cell count: differential count and cell viability assay: smear preparation and staining Labeling techniques in fluorescence microscopy (labeling with antibodies, labeling with toxins and other specific molecules, labeling with substrate and ligand), Isolation and purification of DNA using CTAB method, Gel electrophoresis for DNA visualization, Isolation and purification of total RNA by adsorption and elution method, Amplification of DNA by polymerase chain reaction (PCR), DNA amplification and real time estimation using real time PCR device by SYBR green method, Protein isolation and visualization by Western blotting.

Course Objectives

Course Outcomes

1. To become familiar with cell and molecular biology experiment related equipment <br />2. To learn microscopy techniques <br />3. To learn extraction and purification processes for different biomolecules <br />4. To learn the visualization and quantification processes of different biomolecules <br />5. To learn molecular biology techniques

Essential Reading

Supplementary Reading