The controlled rate freezer allows,
implementation of any desired
temperature vs time protocol. It can be
used both for linear and nonlinear
cooling profiles as per requirement.
When precisely monitored, the
controlled rate freezer offers the
advantage of preventing rapid cellular
dehydration and ice-crystal formation,
leading to high cell viability compared to
conventional techniques. Biological
materials, both live and non-living may
be stored over long perions by cooling
close to -200oC, the temperature of
liquid nitrogen. Unfortunately cells may
die during the freezing process. Cell
damage during freezing may be avoided
by adding cryoprotective chemicals to
the medium and cooling under specific
temperature-time profile