Seminar Title
Molecular mechanism of non-host defence mediated by active protagonists conferring broad spectrum disease resistance against rice blast (Magnaporthe oryzae) by programmed cell death
Seminar Type
Progress Seminar
Department
Life Science
Speaker Name
Reecha Mohapatra ( Rollno : 520ls2005)
Speaker Type
Student
Venue
LS Seminar Room
Date & Time
31 Jan 2023 4:00 PM
Contact
Prof. Binod Bihari Sahu
Abstract

­­­­­Rice blast is a major disease in rice deterring the crop production up to 30% which in turn affects global food security. Several studies on the identification of the disease resistance genes hitherto resistant cultivar did not solve to culminate the disease from the field. However, there are plants in nature that are not infected by the rice blast pathogen and are known to be non-host. A non-host plant provides broad-spectrum disease resistance against a pathogen race. The successive repertoire of effectors actively engages to suppress the pathogen-triggered immunity as well as effector-triggered immunity during non-host resistance (NHR). The infected single cell and neighbouring cells communicate and activate the cell death in an orchestrated fashion. The NHR mechanism results in hypersensitive reactions like oxidative burst and programmed cell death (PCD) at the site of infection that restricts further spreading of the disease into adjacent cells. Although non-host resistance played by various NHR genes is reported, the underlying molecular mechanism of non-host resistance remains elusive. In the preliminary phase of the work, relevant mutants of Arabidopsis were tested and confirmed for homozygosity using molecular markers. Then they were challenged with rice blast and infection dynamics were studied using staining and microscopy. Simultaneously, an ion leakage assay was performed to check the severity of the infection. Thus, the comparative oxidative burst, ion leakage, and cell death study can lead to the identification of the active protagonists from the non-host which in turn would help in accessing the application potential of the same against rice blast disease. Further, the cell death pathway will be studied to delineate its relevance if any in the host.