High Pressure liquid Chromatography
Make & Model: Agilent 1200
Location: Organic Synthesis and Molecular Engineering laboratory, BMBT- 3rd Floor, Chemistry Department
Instrument Incharge: Prof. Debayan Sarkar
In-charge Email Id: sarkard@nitrkl.ac.in
Instrument Status: Functional
Specification: Semi Preparative Agilent 1200 High Pressure Liquid chromatography with Chiral columns
Principle: High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography) is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out of the column.
Application: To ensure product purity, separation of disatereomers and enantiomers