Gradient PCR
Make & Model: Bio-Rad/My CyclerTM
Location: MN-348
Instrument Incharge: Prof. Surajit Das
In-charge Email Id: surajit@nitrkl.ac.in
Instrument Status: Functional
Specification: 96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x 96-well plate
Principle: PCR is a chain reaction, a small fragment of the DNA section of interest needs to be identified which serves as the template for producing the primers that initiate the reaction. One DNA molecule is used to produce two copies, then four, then eight and so forth.
Application: Amplification of DNA fragments
Spinning drop tension meter
Make & Model: Kruss, Site 100
Location: Interfaces and Nanomaterials Lab
Instrument Incharge: Prof. Santanu Paria
In-charge Email Id: sparia@nitrkl.ac.in
Instrument Status: Functional
Specification: Less than 1 mN/m surface tension
Principle: Imaging
Application: Interfacial tension
Muffle furnace
Make & Model: Rani lab, 2012
Location: BMBT-418 ( Dept. of chemistry)
Instrument Incharge: Prof. Priyabrat Dash
In-charge Email Id: dashp@nitrkl.ac.in
Instrument Status: Functional
Specification: temp. - 350 degree C
Principle: to heat the air inside its chamber using the basic fundamentals of thermal convection and thermal radiation.
Application: A muffle furnace is a furnace in which the subject material is isolated from the fuel and all of the products of combustion.
Diffential Scanning Calorimetry
Make & Model: Netzsch, Germany, STA449C/4/MFC/G
Location: MC Lab
Instrument Incharge: Prof. Bibhuti Bhusan Nayak
In-charge Email Id: bbnayak@nitrkl.ac.in
Instrument Status: Non-Functional
Specification: Specification/features
• WorkingTemperature upto 1500oC
• Controlled atmosphere
Principle: Thermal analysis: Simultaneous DSC and TG
Application: Applications
• Determination of phase transition temperature
• Heat of crystallization
• Weight loss in materials on heating
• Specific heat measurement
• Study of reaction kinetics
Programmable Controlled Rate Freezer
Make & Model: Planar Kryo-560-16 & 360-1.7
programmable control rate freezer
cooling down to -180oC.
Location: Tissue Engineering Lab-II (stem cell culture)
Instrument Incharge: Prof.(Ms.) Krishna Pramanik
In-charge Email Id: kpr@nitrkl.ac.in
Instrument Status: Functional
Specification: Freezing of live biological samples in
bags, ampoules and straws,
Forced laminar flow for efficient and
even cooling,
Lower temperature limit: -180oC,
Cooling rates: 0.01 to 50oC/min
Principle: The controlled rate freezer allows,
implementation of any desired
temperature vs time protocol. It can be
used both for linear and nonlinear
cooling profiles as per requirement.
When precisely monitored, the
controlled rate freezer offers the
advantage of preventing rapid cellular
dehydration and ice-crystal formation,
leading to high cell viability compared to
conventional techniques. Biological
materials, both live and non-living may
be stored over long perions by cooling
close to -200oC, the temperature of
liquid nitrogen. Unfortunately cells may
die during the freezing process. Cell
damage during freezing may be avoided
by adding cryoprotective chemicals to
the medium and cooling under specific
temperature-time profile
Application: Preservation of cells & tissues,
Preservation of biological implants &
tissue constructs,
Preservation of sperms, ova and embryos.
Preservation of plant gsmplasm and other
live biological materials,
