Seminar Details

Seminar Title
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Biological synthesis of metal / metal oxide nanoparticles and their applications in food safety
Seminar Type
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Defence Seminar
Department
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Biotechnology and Medical Engineering
Speaker Type
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Student
Speaker Name
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BHASKAR DAS ( RollNo : 515BM1007)
Date  &  Time
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14 Jun 2021  11:00 AM
Venue
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MS Teams [Online code to join - nigc3m3]
Contact
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Prof. Balasubramanian P
Abstract
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The present study focused on the biological synthesis of metal and metal oxide nanoparticles using plant sources as reducing agents and finding their suitable applications in two different aspects of food safety measures (1) antimicrobial activity, and (2) biosensing of food borne pathogens. Two different plant extracts were utilized for the synthesis of metal and metal oxide nanoparticles- Prunus nepalensis (fruit extract for Au NPs) and Bauhinia purpurea (leaf extract for MgO nanoflakes). Then the detailed characterizations of the biogenic Au NPs and MgO nanoflakes were performed by using various sophisticated analysis. Antimicrobial activity of positively charged (+2.70 mV) MgO nanoflakes was investigated against Staphylococcus aureus which demonstrated excellent efficacy as a potential antibacterial agent with a MIC of 250 μg/mL. Another main aim of the study was to utilize biologically synthesized nanoparticles in biosensing applications for the detection of food borne pathogens. Peroxidase mimicking biogenic Au NPs played a significant role in amplifying the detection signal, which could be visualized by the naked eye and could be utilized in further developments of point of care detection assays for various analytes, such as pathogen, disease biomarkers, and toxins for future applications.