Course Details

Subject {L-T-P / C} : CH2471 : Biochemical Engineering Laboratory {0-0-3 / 2}
Subject Nature : Practical
Coordinator : Prof. Susmita Mishra


1. Different sterilization and inoculation techniques.
2. Preparation of various types of media.
3. Effect of pH, Substrate conc. on cell growth.
4. Effect of temperature on cell growth.
5. Determination of volumetric mass transfer co efficient (Kla)
6. Determination of mixing time.
7. Determination of size and density of the microbial cells.
8. Determination of thermal death rate constant.
9. Preparation of immobilized whole cell system.
10. Substrate degradation, cell growth and product formation kinetics using free cells and whole cell immobilization.
11. Treatment of sample waste water in a bioreactor
12. Production of Acetic acid using fermenter.

Course Objectives

  1. The basic objective is to equip the students with various equipments and learn the sterilization and cell separation techniques along with cell growth kinetics.

Course Outcomes

On attending this course the students will be able to:
(i) Handle the biochemical lab equipments independently
(ii) Understand the various sterilization and cell isolation techniques
(iii) Perform experiments on enzyme kinetics

Essential Reading

  1. 1. R. Dutta, Fundamentals of Biochemical Engineering, Springer , 2008
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Supplementary Reading

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