Course Details

Subject {L-T-P / C} : CH6131 : Bioprocess Engineering {3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Susmita Mishra


Bioprocess principles kinetics of biomass production, substrate utilization and product formation Batch and Continuous culture, Fed batch culture. Introduction: Fermentation Processes General requirements of fermentation processes, An overview of aerobic and anaerobic fermentation processes and their application in industry, Medium requirements for fermentation processes Thermal death kinetics of micro-organisms, Heat Sterilization of liquid media, Filter sterilization of liquid media and air. Enzyme Technology, Microbial metabolism enzymes: Classification and properties, Applied enzyme catalysis - Kinetics of enzyme catalytic reactions, Metabolic pathways, Protein synthesis in cells. Bioreactor design & operations, Selection, scale-up, operation of bioreactors, Mass transfer in heterogeneous biochemical reaction systems Oxygen transfer in submerged fermentation processes oxygen uptake rates and determination of oxygen transfer rates and coefficients role of aeration and agitation in oxygen transfer, Heat transfer processes in biological systems, Recovery and purification of products. Introduction to instrumentation and process control in bioprocesses: measurement of physical and chemical parameters in bioreactors, Monitoring and control of dissolved oxygen, pH, impeller speed and temperature in a stirred tank fermenter. Bioprocess patenting and economics.

Course Objectives

  1. This course would help to design, build, control, and operate bioreactors and fermenters.
  2. It would also equip the students with basic concepts and prepares them to meet the challenges of the new and emerging biotechnology industry
  3. To provide a platform to understand the biological processes kinetics in order to apply them effectively

Course Outcomes

1. To grow acumen in Biochemical engineering process development and design.
2. To understand the concept of Microbiology and their application
3. To understand the concept of enzyme kinetics
4. To help in design of batch bioreactor and scale up a process
5. To enable one to work in bio-product manufacturing, pharmaceutical companies and R&Ds

Essential Reading

  1. J.E. Bailey and D.F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill, Publishing , 1986
  2. M.L. Shuler and F. Kargi, Bio-process Engineering, Prentice Hall , 2002

Supplementary Reading

  1. Rajiv Dutta, Fundamentals of Biochemical Engineering, 1st Edition, Springer , 2008
  2. John Villadsen, Jens Nielsen, Gunnar Lidén, Bioreaction Engineering Principles, Springer Science & Business Media , 2011