राष्ट्रीय प्रौद्योगिकी संस्थान राउरकेला
National Institute of Technology Rourkela

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Seminar Title
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Modulation of Mechanical Properties of Host Membrane by Hepatitis E Virus Like particle (HEV-LP) During Its Entry
Seminar Type
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Departmental Seminar
Department
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Life Science
Speaker Type
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Student
Speaker Name
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Ashutosh Prince
Date  &  Time
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11 Feb 2019  9:00AM
Venu
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LS Seminar Room (Library annexe, Ground Floor)
Contact
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Prof. B.B. Sahu
Abstract
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Hepatitis E virus is responsible for over 20 million viral infections globally. Although specific HEV receptors are yet to be identified but the entry of HEV has been proposed to occur both by endocytosis as well as direct membrane fusion. Host cell membrane remodeling is critical during the entry of any virus and would involve significant lipid organization and local to global changes in the host cell membrane properties in order to internalize the virus. Here, we reconstitute the host-virus interaction using hepatic cell model membrane and HEV-LP to understand the membrane interactions of HEV. Using reconstitution, fluorescence microscopy and spectroscopy, we find that binding of HEV-LPs results in significant change in the dipole potential and membrane fluidity in hepatic cell model membrane, wherein, lipids containing phosphatidylcholine heads and sphingomyelin are critical for the binding and membrane internalization of HEV in addition to low cholesterol content. Interestingly, HEV-LP crowding on membranes containing phosphatidylserine, and phosphatidyl glycerol head group containing lipids decreases the membrane fluidity, on the contrary, it was found to enhance the fluidity of membranes containing phosphatidylethanolamine and phosphoionositol head group containing lipids. Furthermore, using lipid monolayer isotherms we experimentally quantified the modulation of in-plane elasticity, lipid packing, bending rigidity and penetrability of single membrane leaflet by HEV-LP crowding. Finally, the observed changes in mechanical properties of the host model membranes was correlated to experimentally extracted thermodynamic parameters such as Gibb’s free energy, interaction parameter and enthalpy of mixing of HEV-LP and membrane monolayers. Together, our findings suggest that the changes in the host cell membrane mechanical properties induced by HEV-LP crowding might facilitate virus penetration through host cell membranes. Keywords: Virus-host membrane interaction; Membrane elasticity; Compressibility; Membrane fluidity; Lipid packing.
 
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